13th MicRO Alliance Meeting

第13回京都大学-ミシガン大学-フライブルク大学 3大学連携シンポジウム


The 13th MicRO Alliance Meeting

7-8 November, 2016
Meeting venue: Kyoto University (7 Nov.: Katsura campus, 8 Nov.: Yoshida campus)


【Sponsored by】

Nanotech Career-up Alliance(Nanotech CUPAL)CUPAL(https://nanotechcupal.jp/



京都大学大学院工学研究科附属桂インテックセンター ナノミクス研究部門(http://nanofrontier.kic.t.kyoto-u.ac.jp/


Technical program schedule


◆ 7 November: Katsura Campus, Katsura Hall, B cluster

【MAP @ No.14: Click

Opening Address
Osamu Tabata (Kyoto University)


The University of Michigan Engineering Research Center for Wireless Integrated Micro Sensing and Systems: an Overview
Yogesh B. Gianchandani (University of Michigan)
The mission of the Center for Wireless Integrated MicroSensing and Systems (WIMS2) at the University of Michigan, Ann Arbor, is to advance the investigation, development, and application of sensor-enabled microsystems through basic research, education, and interactions with industry. Created in 2000 with support from the National Science Foundation, the Center brings together research in core technologies (such as MEMS, micro-power circuits, and RF technology) to facilitate microsystems for healthcare, environmental monitoring, and infrastructure monitoring. This presentation will provide a brief overview of WIMS2 and focus more specifically on selected topics related to sensors and actuators for implantable biomedical devices and for air quality monitoring.


Three-dimensional SU-8 Microstructures by Single-exposure Projection Lithography: Technology, Applications and Simulation
Oliver Paul (Freiburg University) and Yoshikazu Hirai (Kyoto University)
Ultraviolet single-step projection lithography was recently found to be a convenient technique to fabricate complex three-dimensional (3D) microstructures in thick SU-8 photoresist. It has been used to fabricate regular arrangements of containers connected by single or multiple buried microchannels of various lengths. The purpose of these structures is to enable the geometrically controlled growth of connected cell tissues in predefined geometries, for fundamental cell interaction studies and lab-on-a-chip applications. Unlike conventional photolithography, where the exposure essentially translates the mask pattern vertically into the photoresist, in projection lithography the high numerical aperture of the projection objective causes the resist to be exposed to a 3D intensity depending on (i) the mask pattern, (ii) the exposure dose and (iii) the focus depth within the resist. In order to model the exposure outcome due to a mask and, even better, to predict the mask geometry required for a wanted 3D microstructure, novel simulation methods are needed. We propose a practical simulation system solving this problem and predict 3D profiles resulting from single-step projection lithography. This simulation consists of exposure, post-exposure bake process (PEB), and development modules for SU-8 photoresist. The simulation results are compared with the experimental results, and the simulation algorithm is validated.


TEMPURA – Teeth Measurement and Profiling using Replicated Advanced Optics
Phuong Ha Cu Nguyen (Freiburg University)
In the dental community, there is a considerable demand for a fast, comfortable yet rapid means for a complete characterization of a patient’s teeth before, during and after treatment. We will present here concepts in the the development and fabrication of a micro-optics-based 3-dimensional (3D) imager for high-resolution measurement of teeth profiles using a far-field patterned-light micro-projector and a high depth-of-field camera system. Based on the active triangulation method, the structured light is projected onto a 3D shaped surface by a micro-projector while the geometry of the distorted patterns is observed via the camera system from a different perspective. The imaged surface, including possible cavities, may result in a large depth variation of 4-20 mm. The camera system is designed as dual-lens array with two different focal lengths to image the projected pattern over the large distal surface. The proposed imaging system would be produced in a highly-miniaturized package, offering a highly precise 3D image without requiring high accuracy in handling.




Engineered Biointerfaces through Tailormade Surface-attached Polymer Networks – From to new diagnostic tools to implantable materials
Jürgen Rühe (Freiburg University)
Surface-attached polymer networks can help to tailor the surface properties of a material precisely to the specific needs laid out by medical applications and to create a favorable interaction of the artificial material with the biological system. However, chemical tools are needed, which allow for the attachment of polymer molecules to the device surfaces to ensure sufficient stability of the coating. Our approach for such surface architectures is based on polymers bearing photochemically or thermally reactive groups. Upon activation these groups attach to neighboring C,H-groups via C,H insertion crosslinking (CHic). Surface-attached polymer networks can be generated through this process and deposited on different substrate materials ranging from biomaterials, polymers to glass or metal surfaces. These specifically designed surfaces can be used for a variety of biomedical applications. We use such coatings in blood contacting environments in the context of ventricular assist devices and for the reduction of scar formation after a glaucoma treatment. If further functional groups are incorporated, such layers can also take on an active role in the interaction with a biomedical environment and can be used to increase the sensitivity of biochips for DNA, RNA and protein or cell analysis. In the latter case we use such systems for the capture and analysis of circulating tumor cells (CTC). Additionally, we describe the development of new biochips which allow e.g. a diagnosis of HPV infections or the detection of mastitis in milk samples. In many cases the combination of a bioinert background and very specific probes are key to allow for a precise and reliable analysis especially in real serum samples.


Nano-plasmonic Thin Films for Controlling Marangoni Flows Around a Microbubble
Kyoko Namura and Motofumi Suzuki (Kyoto University)
Thermoplasmonic effect of gold nanoparticles provides a powerful local heat source, which opens up new approaches to microfluidic control by thermal methods. Here, we demonstrate the self-assembling of nano-plasmonic thin films by using a dynamic oblique deposition technique and their capability for thermoplasmonic microfluidic control. Owing to strong interference, optical absorption to gold nanoparticles layer of those films is enhanced up to 0.97. The highly localized optical absorption and subsequent heat generation in the thin gold nanoparticles layer (∼10 nm) realize strong temporal modulation of local fluid temperature, which was revealed through photoacoustic measurements. The nano-plasmonic thin films are then used to demonstrate the microfluidic control based on Marangoni effect. Marangoni effect induces fluid motion because of the surface tension differential under temperature gradient along a gas-liquid surface. Taking advantage of highly localized heat generation of the thermoplasmonic effect, a microbubble is generated in water by focusing a laser onto the film. Furthermore, temperature gradient around the bubble is controlled by tuning the laser spot position and power, and induces rapid Marangoni vortex flow, which was found to be useful for mixing, collecting, sorting, and focusing particles dispersed in water.


Nanoplasmonic Biosensing for Immune Status Monitoring
Katsuo Kurabayashi (University of Michigan)
Recent advances in nanomaterials and nanofabrication have brought nanoplasmonic biosensing technologies that show great promise in providing fast, real-time label-free detection of biological species. This talk summarizes our recent study developing new metrology techniques that incorporate nanoplasmonic biosensors for imaging-based multiplexed protein assay. Our study aims at the translation of nanoplasmonic biosensing to clinical applications as well as fundamental single-cell study. The biosensors for clinical diagnostics are based on arrayed gold nanorod ensemble structures constructed by a scalable microfluidic channel patterning technique. “Nanoplasmonic biosensor antenna,” each consisting of a synthetic nucleic acid-linked nanostructures, are synthesized for single-cell protein secretion measurement.


Coffee Break


Concurrent MR and Optical Microscopy using Adaptive Optics
Matthias Wapler and Ulrike Wallrabe (Freiburg University)
While microscopic magnetic resonance imaging can produce three dimensional images e.g. of biological tissue and may also provide information of the chemical position, its resolution is limited to around 10µm and it requires long imaging times. Optical microscopy, in contrast, can achieve sub-µm resolution with real-time imaging. In this talk, I will present a microscope that can operate inside a preclinical 9.4T MR scanner and acquire concurrent MR and optical images to combine the advantages of both methods. After an overview over the major issues of MR compatibility, the overall design of the microscope and material aspects, I will present in detail the development of a compact adaptive lens that provides the focusing. Finally, I will give an outlook on ongoing work to integrate optical microscopy into high-field MR imaging and spectroscopy devices at 11.4T.


Microfluidic Devices for Biophysical Studies and Engineering Applications of Motor Proteins
Ryuji Yokokawa (Kyoto University)
Micro/nano fabrication technologies have been prevailed to many research disciplines. Due to the size matching between fabrications and biomaterials, cells and tissues have been targets of researchers in engineering fields. In contrast, although fabrication technologies have already reached to nanometer scale and can be applied to even a single molecule, applications with biomolecules are still limited. Expecting that such applications will provide a powerful assay platform at molecular level, our group has been focusing on the applicability of microfluidic devices to motor proteins. In this presentation, I will overview our two research directions: one is to construct nanoscale systems that cannot be realized only by top-down micro/nano fabrications, and the other is to propose experimental tools for biophysical studies. As an example for the former approach, a molecular reaction system and a molecular separation system driven by kinesin and dynein motors are introduced. For the latter approach, the tug-of-war of microtubules by kinesin and dynein motors and a microfluidic device to evaluate active transport of kinesins will be presented. We have proposed several bioassays that create functional nanoscale systems and contribute to understanding of in vivo functions of motor proteins. As an application of micro/nano fluidic devices, we keep exploring how engineering approaches can deepen science at molecular scale.


Microfluidic Platforms for Single Cell Heterogeneity Studies in Cancer
Euisik Yoon (University of Michigan)
Microfluidic platform technologies have been developed for single cell analysis in cancer prognosis and treatment. Cancer drug screening devices targeting for cancer stem-like cells will be presented along with a few microfluidic chips for correlating phenotypic behaviors to genetic analysis toward personalized medicine.


TOFU – Tubular Optofluidics
Hans Zappe (Freiburg University)
We will present a new direction in three-dimensional liquid optical systems, tubular optofluidics. Through this new approach, high-quality optical imaging and beam formation is becoming possible using fluidic components. Also interesting in this regard are concepts such as high-contrast liquid-based tunable irises or variable slit apertures; hydraulically-actuated fluid-filled membrane lenses with aberration free tuning; or tubular fluidic systems allowing controlled variation of astigmatism. These examples are compact, self-contained devices some of which have been integrated into adaptive imaging systems in which tuning is accomplished using only liquids and soft-matter materials, suggesting entirely new approaches for realization of complex optical microsystems.





◆ 8 November: Yoshida Campus, International Innovation Center Symposium Hall

【MAP @ No.69: Click


●CUPAL special session: Biological function analysis based on informatics

Kentaro Kawai (Osaka University)
Bioinformatics has play an important role of many areas of biology, such as genomics, proteomics, transcriptomics, and metabolomics over the last decade. Next-generation DNA sequencers are creating a huge amount of raw DNA sequence data that have to be analyzed and associate with genomes, reference maps, RNAs, proteins, chemicals, pathways, systems, and structures. State-of-the-art biological function analysis requires data mining from the enormous digitalized data, that only can be done by experts understanding both field of biology and computer science. A cooperation with bioinfromaticians is one of key factors to success in exploring the life phenomena. The lecturers give different aspects of bioinformatic analysis on each objective organisms from the basics.


Multi-Omics Strategy to Unveil the Molecular Mechanisms of Non-Classical Model Organisms
Kazuharu Arakawa (Keio University)


DNA Methylation and Metabolic Disturbances: Beyond Genetics
Marie Loh (National University of Singapore (NUS) and A*STAR)


Digitalizing Transcription by Single Cell Transcriptomics
Akira Watanabe (Kyoto University)




●2nd French-Japanese Workshop on Micro & Nanotechnology